pSKI074 map

by admin — last modified Apr 10, 2008 09:50 AM

  • click for sequence

  • Activation-tagging vector constructed by Igor Kardailsky in the Weigel lab (Weigel et al., 2000). The complete sequence has been deposited in GenBank under accession AF218466.
  • The CaMV 35S enhancers in these vectors correspond to nucleotides -417 to -86 relative to the transcription start.
  • Kanamycin gene for selection on MS media.
  • Recommended Agrobacterium strain is GV3101 pMP90RK. Host and helper plasmid markers are kanamycin, gentamycin and rifampicin resistance.
  • Plasmid selection in E. coli and in A. tumefaciens is ampicillin resistance.
  • Sites that leave the pBstKS+ sequences intact and cut only on one side between pBstKS+ and either left or right border can be used for plasmid rescue. Note that there are additional BssS1 sites not shown on the map.
  • NOTE: The multimerized 35S enhancers tend to be unstable in Agrobacterium. Always inoculate cultures for transformation from plates that have been streaked from -80°C stocks.



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