lfy Alleles

by admin — last modified Mar 27, 2008 03:47 PM

Weigel Lab - leafy mutant alleles

The list below shows information on 30 alleles. Reference alleles are lfy-6 (strong) and lfy-5 (weak) in Landsberg erecta, and lfy-1 or lfy-12 (strong) and lfy-2 (weak) in Columbia. If you want to obtain mutant seeds, please contact the stock center (see right most column).

Several alleles have the same nucleotide change, and the three strong reference alleles as well as the two weak reference alleles have the same sequences.

You may also want to consult the page on CAPS markers for genotyping information.

The LEAFY sequence can be found in GenBank under accession M91208. The numbering refers to the longest splice form, which produces a 424 aa protein.

Allele

Ecotype

Isolation number

Ref.

Source

Mutagen

Class

Nucleotide sequence

Amino acid sequence

NASC stock
lfy-1

Col-0

   

1,3

Haughn

EMS

strong

C94T

Q32stop

CS6228

lfy-2

Col-0

   

2,3

 Haughn

EMS

weak

C719T

P240L

CS6229

lfy-3

L-er

S192

3

 Smyth

EMS

intermed.

C731T

T244M

CS6273

lfy-4

L-er

S679

3

Smyth

EMS

intermed.

C712A

E238K

CS6274

 lfy-5

L-er

S1251

3

Smyth

EMS

weak

C719T

P240L

CS6275

 lfy-6

L-er

S1339

3

Smyth

EMS

strong

C94T

Q32stop

 CS8552
 lfy-7

Ws-0

993

3

Feldmann

T-DNA

strong

C569T

Q187stop

CS6277
 lfy-8

L-er

188

3

Poethig

EMS

strong

C94T

Q32stop

 
 lfy-9

Col-0

278

3

Poethig

EMS

intermed.

G992A

R331K

CS6278
 lfy-10

Col-0

S2380

3,6

Alvarez

EMS

weak

C719T

P240L

CS6279
 lfy-11

L-er

S2143

3,6

Alvarez

EMS

strong

C355T

Q187stop

 
 lfy-12

Col-0

 

4,6

Huala

EMS

strong

C94T

Q32stop

 
 lfy-13

Col-0

 

4,6

Huala

EMS

strong

G1032A

W344stop

CS6281
 lfy-14

Col-0

 

4,6

 Huala

EMS

strong

C796T

Q266stop

CS6282

lfy-15

Col-C24

T1

3

Perez

T-DNA

strong

Insertion after A457

Rearrangement after Q196

CS6283

lfy-16

Col-0

 

5,6

Meeks-
Wagner

EMS

strong

AGGT>TGGT

2nd splice acceptor

 
lfy-17

L-er

 

6

Hülskamp

EMS

strong

G1128A

W376stop

 
lfy-18

L-er

10567

6

 Conway

DEB

strong

A334T

R112stop

 
lfy-19

L-er

11125

6

Conway

DEB

strong

A inserted after A1029

Frameshift after S343

 
lfy-20

L-er

10996

6

Conway

DEB

weak

A916G

N306D

 
lfy-21

Col-gl1


6

 Morris

EMS

strong

C355T

Q111stop

CS6284

lfy-22

L-er

63.1

6,7

 Jack

EMS

very weak

G209A

Gly70D

CS6285

lfy-23

L-er


6

Alvarez

DEB

strong

C792A

Y264stop

 
lfy-24

L-er

phyB#3

6

Weigel

EMS

strong

C583T

Q195stop

 
lfy-25

L-er (in lfy-22)

eol23.1

6

Weigel

EMS

strong

G767A

Gly256D

 
lfy-26

L-er

S1339

8

 Weigel

EMS

strong

G1128A

Trp376stop

 CS8553
lfy-27

L-er

48-2C

6

Jack

EMS

strong

C490T

Q164stop

 
lfy-28

L-er

683

6

Fletcher

EMS

strong/
intermed.

C923T

P308L


lfy-29

L-er (in lfy-22)

eol31.1

6

 Weigel

EMS

strong

C97T

Q33stop

 
lfy-30

Ws-0

 

6,9

Lee

T-DNA

strong

deletion

   

References:

1 E. A. Schultz & G. W. Haughn (1991) Plant Cell 3, 771-781.


2 E. A. Schultz & G. W. Haughn (1993) Development 119, 745-765.


3 D. Weigel et al. (1992) Cell 69, 843-859.


4 E. Huala & I. M. Sussex (1992) Plant Cell 4, 901-913.


5 S. Shannon & D. R. Meeks-Wagner (1993) Plant Cell 5, 639-655.


6 A. Maizel et al. (2005) Science 308, 260-263.
7 J. Z. Levin & E. M. Meyerowitz (1995) Plant Cell 7, 529-548.


8 I. Lee, D. S. Wolfe, O. Nilsson & D. Weigel (1997) Current Biology 7, 95-104.


9 A. Sessions, A., M. F. Yanofsky, & D. Weigel (2000) Science 289, 779-781.

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