CAPS Markers
| forward primer | reverse primer | enzyme | fragments in bp |
purpose |
| TTT AAC GCT CAT CCT CCA CTC TCT ATT TG | TTA ATT AGG ATA ACG AC |
Xcm1 |
220 (wt) 191, 19 (lfy-26) |
genotype lfy-26 |
| TAT GGA TCC TGA AGG TTT CAC G | CGG GCA TAG AAA TGT TG |
Mae3 |
434, 103 (wt) 434, 93, 10 (lfy-6, lfy-12) |
genotype lfy-6 and lfy-12 |
| CGC TCA GTT GGT TGA CTC CGA CTC | GCT GAT ATC GTT TAA CTA TCT TAA GAT ACA TGG |
Rsa1 |
970, 813, 156 (L-er) 813, ~600, ~370, 156 (No-0) |
mapping |
| CTG TCA ATT TCC CAG CAA GAC AC | TAG TCA CGT GAA CAT ATA CAT AC |
Rsa1 |
559, 124, 110, 36 (L-er) 412, 147, 124, 110, 36 (Col) |
mapping |
| AAG CAG CCG TCT GCG GTG TCA GCA GCT GTT | CTG TCA ATT TCC CAG CAA GAC AC |
BstAP1 |
345, 25 (wt) 370 (lfy-6, lfy-12) |
genotype lfy-6 and lfy-12 |
| forward primer | reverse primer | enzyme | fragments in bp |
purpose |
| CAC CTC CTC TTC TTG AC | GGG TTA TGT TTT TGA GTT TGA TGG |
Afl3 |
1274, 84, 57 (wt) 703, 571, 84, 57 (ufo-2) |
genotype ufo-2 |
| GTG GCG GTT CAG ACG GAG AGG | AAG GCA TCA TGA CTG TGG TTT TTC |
Taq1 |
983, 316 (Col-0) ~600, ~383, 316 (L-er) |
mapping |
| TAT GAT CTT TGC CTC TTT TCC TAC | GGG TTA TGT TTT TGA GTT TGA TGG |
BsiE1 |
1706 (L-er) 1433, 273 (Col-0, No-0, Ws-0) |
mapping |
|
For CAPS markers, see Konieczny A, Ausubel FM: A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 1993, 4:403-410.
dCAPS stands for Derived CAPS; one primer contains a mismatch, such that a new restriction site that spans the primer and the amplified sequence is generated. See Neff MM, Neff JD, Chory J, Pepper AE: dCAPS, a simple technique for the analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J. 14:387-395. |
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