READ ME - Development

by admin — last modified Mar 25, 2008 03:24 PM

To cite data from the developmental set, please refer to: Schmid, M., Davison, T. S., Henz, S. R., Pape, U. J., Demar, M., Vingron, M. Schölkopf, B. Weigel, D., and Lohmann, J. (2005) A gene expression map of Arabidopsis development. Nature Genetics, 37, 501-506. Download here.

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AtGenExpress Developmental series (triplicate arrays, Columbia background)

RNAs provided by Markus Schmid and Jan U. Lohmann (MPI Tuebingen)
Bernd Weisshaar (MPI Cologne; seed & silique development)
Ben Scheres (Univ. Utrecht; development on MS agar)
Dave Twell (Univ. Leicester; pollen)
Scott Poethig (Univ. Pennsylvania ; phase change)
CAGE consortium (common reference samples)

Probes prepared and hybridized by Markus Schmid, Jan Lohmann and Monika Demar at the MPI Tuebingen (Dept. Weigel) 
according to ATGE Probe Synthesis Guidelines.

Washing and scanning were performed on Affymetrix Fluidics Station 450 and Affymetrix GeneChip Scanner 3000 
following standard Affymetrix protocols.

Normalization and expression estimate computation were carried out using gcRMA implemented in R using standard settings. 
Output values are log2.

For publications, please refer to

Schmid, M., Davison, T. S., Henz, S. R., Pape, U. J., Demar, M., Vingron, M. Sch�lkopf, B. Weigel, D., and Lohmann, J. (2005) A gene expression map of Arabidopsis development. Nature Genetics, accepted for publication.
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